EF1a-Cas9-2XNES-2ERT2
(Plasmid #120551)

• Purpose: Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells
• Depositing Lab: Yu Wang
• Publication: Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:208-219. doi: 10.1016/j.omtn.2018.08.022. Epub 2018 Sep 1.

Backbone

• Vector backbone: pRRL.sin-18.ppy.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9, NES and ERT2
• Species: Synthetic
• Promoter: EF1α

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • EF1a-Cas9-2XNES-2ERT2.gb
• A portion of this plasmid was derived from a plasmid made by: Cas9 was cloned from Addgene plasmid #42230; ERT2 was cloned from the pAd-CreER plasmid (a gift from T. C. He’s lab, Chicago University); NES sequences were synthesized.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry