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Addgene

pAM5187
(Plasmid #120086)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120086 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAM4787
  • Backbone size w/o insert (bp) 6194
  • Vector type
    other

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pAM4787 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
  • Species
    Synechococcus elongatus PCC 7942
  • Insert Size (bp)
    148

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM5187 was a gift from Susan Golden (Addgene plasmid # 120086 ; http://n2t.net/addgene:120086 ; RRID:Addgene_120086)

  • For your References section:

    Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Chen Y, Taton A, Go M, London RE, Pieper LM, Golden SS, Golden JW. Microbiology. 2016 Dec;162(12):2029-2041. doi: 10.1099/mic.0.000377. Epub 2016 Oct 14. 10.1099/mic.0.000377 PubMed 27902432
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