pAM5057
(Plasmid
#120085)
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PurposeBroad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 120085 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRSF1010Y25F-AADA
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin and Streptomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter
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SpeciesBroad range of genetically tractable cyanobacteria
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM5057 was a gift from Susan Golden (Addgene plasmid # 120085 ; http://n2t.net/addgene:120085 ; RRID:Addgene_120085) -
For your References section:
Regulation of gene expression in diverse cyanobacterial species by using theophylline-responsive riboswitches. Ma AT, Schmidt CM, Golden JW. Appl Environ Microbiol. 2014 Nov;80(21):6704-13. doi: 10.1128/AEM.01697-14. Epub 2014 Aug 22. 10.1128/AEM.01697-14 PubMed 25149516
