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Addgene

pAM4951 (pCV0063)
(Plasmid #120084)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 120084 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    S7942NS1-RK2BOM_aadA_nt_ccdB-SwaI
  • Backbone size w/o insert (bp) 5008
  • Modifications to backbone
    Plasmid assembled from 3 CYANO-VECTOR modular devices: pCVD019 / CV-S7942NS1-RK2BOM: The device comprises the chromosome integration site NSI for Synechococcus elongatus PCC7942. The device has an origin of replication for E. coli derived from pBR322 and an oriT site for conjugal transfer recognized by the RK2/RP4 mobilization machinery. pCVD065 / CV-aadA_nt: The device confers spectinomycin and streptomycin (SpSm) resistance. About hundred nts of the omega cassette follow the CDS but it does not have a synthetic terminator. Nevertheless this device works well. pCVD015 / CV-ccdB-SwaI: The device comprises the ccdB gene (toxic for commonly used E. coli cell line), which can be taken out with the SwaI endonuclease and swapped for the sequence(s) of interest using adaptors optimized for seamless assembly reaction.

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
  • Species
    Synechococcus elongatus PCC 7942

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM4951 (pCV0063) was a gift from Susan Golden (Addgene plasmid # 120084 ; http://n2t.net/addgene:120084 ; RRID:Addgene_120084)
  • For your References section:

    Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria. Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. Nucleic Acids Res. 2014 Jul 29. pii: gku673. 10.1093/nar/gku673 PubMed 25074377