pAM4941 (pCV0053)
(Plasmid #120083)

• Purpose: Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).
• Depositing Lab: Susan Golden
• Publication: Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku673.

Backbone

• Vector backbone: S7942NS3-RK2BOM_aacC1_ccdB-SwaI
• Backbone size w/o insert (bp): 4705
• Modifications to backbone: Plasmid assembled from 3 CYANO-VECTOR modular devices: pCVD025 / CV-S7942NS3-RK2BOM: The device comprises the chromosome integration site NSIII for Synechococcus elongatus PCC7942. The device has an origin of replication for E. coli and a bom site for conjugal transfer, which are derived from pBR322. The device also comprises a Tetracycline resistance marker. pCVD001 / CV-aacC1: The device confers gentamicin (Gm) resistance. pCVD015 / CV-ccdB-SwaI: The device comprises the ccdB gene (toxic for commonly used E. coli cell line), which can be taken out with the SwaI endonuclease and swapped for the sequence(s) of interest using adaptors optimized for seamless assembly reaction.
• Vector type: other

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
• Species: Synechococcus elongatus PCC 7942

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pAM4941 (pcv0053).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry