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Addgene

sup35-R320I
(Plasmid #1199)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS306
  • Backbone manufacturer
    Hieter
  • Backbone size w/o insert (bp) 4381
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sup35p
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2057
  • Mutation
    changed arg 320 to isoleucine
  • GenBank ID
    NC_001136
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

yeast integration plasmid for replacement of Sup35 with mutant. Cut with AgeI for integration. Additional Reference: Bradley ME, Bagriantsev S, Vishveshwara N and Liebman SW. Guanidine reduces stop codon read-through caused by missense mutations in SUP35 or SUP45. Yeast 20, 625-632 (2003).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sup35-R320I was a gift from Susan Lindquist (Addgene plasmid # 1199 ; http://n2t.net/addgene:1199 ; RRID:Addgene_1199)

  • For your References section:

    Epigenetic regulation of translation reveals hidden genetic variation to produce complex traits. True HL, Berlin I, Lindquist SL. Nature 2004 Sep 9;431(7005):184-7. Epub 2004 Aug 15. 10.1038/nature02885 PubMed 15311209
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