pGEM-myr-tdTomato-SV40pA-FKF
(Plasmid
#119867)
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PurposeDonor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T
- Backbone size w/o insert (bp) 3121
- Total vector size (bp) 6408
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemyr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3287
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Tag
/ Fusion Protein
- 2x myristoylation (N terminal on insert)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-myr-tdTomato-SV40pA-FKF was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 119867 ; http://n2t.net/addgene:119867 ; RRID:Addgene_119867) -
For your References section:
Functional Heterogeneity within the Developing Zebrafish Epicardium. Weinberger M, Simoes FC, Patient R, Sauka-Spengler T, Riley PR. Dev Cell. 2020 Mar 9;52(5):574-590.e6. doi: 10.1016/j.devcel.2020.01.023. Epub 2020 Feb 20. 10.1016/j.devcel.2020.01.023 PubMed 32084358