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PurposeA-to-G base editor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 119814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV with a BR322 origin
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameecTadA-ecTadA*(7.10)-nSaCas9
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SaABEmax was a gift from David Liu (Addgene plasmid # 119814 ; http://n2t.net/addgene:119814 ; RRID:Addgene_119814) -
For your References section:
Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Huang TP, Zhao KT, Miller SM, Gaudelli NM, Oakes BL, Fellmann C, Savage DF, Liu DR. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. 10.1038/s41587-019-0134-y PubMed 31110355