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Addgene

pH-A3A-PBE
(Plasmid #119774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAMBIA1300
  • Backbone size w/o insert (bp) 13061
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APOBEC3A-nCas9-NLS-UGI-NLS
  • Alt name
    APOBEC3A-nCas9-UGI
  • Insert Size (bp)
    5106
  • Mutation
    nCas9 has a mutation D10A in wild type SpCas9
  • Promoter maize Ubiquitin-1
  • Tag / Fusion Protein
    • NLS (flanking UGI)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are differences between the Addgene full plasmid sequence and the depositor's reference sequence. These differences do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH-A3A-PBE was a gift from Caixia Gao (Addgene plasmid # 119774 ; http://n2t.net/addgene:119774 ; RRID:Addgene_119774)

  • For your References section:

    Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C. Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. 10.1038/nbt.4261 PubMed 30272679
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