pGEX-4T1 14-3-3 epsilon K49E
(Plasmid #11945)

• Depositing Lab: Michael Yaffe
• Publication: Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Backbone

• Vector backbone: pGEX-4T1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ywhae
• Alt name: 14-3-3 epsilon
• Species: R. norvegicus (rat)
• Mutation: K49E
• Entrez Gene: Ywhae (a.k.a. 14-3-3e)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: SmaI (unknown if destroyed)
• 5′ sequencing primer: pGEX_5
• 3′ sequencing primer: pGEX_3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry