pET28-His-TEV-NQO1
(Plasmid #119058)

• Purpose: For expression in E.coli and affinity purification of N-terminally His tagged NQO1
• Depositing Lab: Yosef Shaul
• Publication: Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5535-40. doi: 10.1073/pnas.0501828102. Epub 2005 Apr 4.

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21 for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NAD(P)H dehydrogenase, quinone 1 (NQO1)
• Species: H. sapiens (human)
• Insert Size (bp): 884
• GenBank ID: NM_000903.2
• Entrez Gene: NQO1 (a.k.a. DHQU, DIA4, DTD, NMOR1, NMORI, QR1)
• Promoter: T7
• Tag / Fusion Protein:
  • 6His, TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: PET REV 5' - GCT AGT TAT TGC TCA GCG G - 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry