Skip to main content
Addgene

pET28-His-TEV-NQO1
(Plasmid #119058)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 119058 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NAD(P)H dehydrogenase, quinone 1 (NQO1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    884
  • GenBank ID
    NM_000903.2
  • Entrez Gene
    NQO1 (a.k.a. DHQU, DIA4, DTD, NMOR1, NMORI, QR1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6His, TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer PET REV 5' - GCT AGT TAT TGC TCA GCG G - 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His-TEV-NQO1 was a gift from Yosef Shaul (Addgene plasmid # 119058 ; http://n2t.net/addgene:119058 ; RRID:Addgene_119058)

  • For your References section:

    Inhibition of NAD(P)H:quinone oxidoreductase 1 activity and induction of p53 degradation by the natural phenolic compound curcumin. Tsvetkov P, Asher G, Reiss V, Shaul Y, Sachs L, Lotem J. Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5535-40. doi: 10.1073/pnas.0501828102. Epub 2005 Apr 4. 10.1073/pnas.0501828102 PubMed 15809436
Related items:
Commonly requested with: