pET-SOMA
(Plasmid #118935)

• Purpose: Used to express the SOMA FRET sensor in E. coli.
• Depositing Lab: Patrick Krysan
• Publication: Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164.

Backbone

• Vector backbone: pET-32(a)+
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
• Alt name: SOMA FRET Sensor
• Species: A. thaliana (mustard weed), Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • Data S1.gb
  • Snap Gene File for pET-SOMA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki. Please note there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.