pMX-eSOX2NRR
(Plasmid
#118825)
-
PurposeRetroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 118825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMX
-
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameeSOX2NRR
-
Alt nameSox2
-
SpeciesM. musculus (mouse)
-
MutationNRR: E46N, I53R, K57R
-
Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMX-eSOX2NRR was a gift from Ralf Jauch (Addgene plasmid # 118825 ; http://n2t.net/addgene:118825 ; RRID:Addgene_118825) -
For your References section:
Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. Veerapandian V, Ackermann JO, Srivastava Y, Malik V, Weng M, Yang X, Jauch R. Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2. 10.1016/j.stemcr.2018.07.002 PubMed 30078555