pFLAG-CrmA
(Plasmid
#11831)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 11831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFLAG
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCrmA
-
SpeciesCowpox virus
-
Insert Size (bp)1100
-
Entrez GeneCrmA (a.k.a. CPXV207)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
WT CrmA in a pFLAG vector, no cloning sites to release just coding frame.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFLAG-CrmA was a gift from Guy Salvesen (Addgene plasmid # 11831 ; http://n2t.net/addgene:11831 ; RRID:Addgene_11831) -
For your References section:
FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens. Muzio M, Salvesen GS, Dixit VM. J Biol Chem. 1997 Jan 31. 272(5):2952-6. 10.1074/jbc.272.5.2952 PubMed 9006941