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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11829 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-23 b
- Backbone size w/o insert (bp) 3666
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaspase 9
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SpeciesH. sapiens (human)
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Insert Size (bp)1373
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Entrez GeneCASP9 (a.k.a. APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56)
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Don't Know (unknown if destroyed)
- 3′ cloning site Don't Know (unknown if destroyed)
- 5′ sequencing primer T7 terminal primer (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET23b-Casp9-His was a gift from Guy Salvesen (Addgene plasmid # 11829 ; http://n2t.net/addgene:11829 ; RRID:Addgene_11829) -
For your References section:
Caspase-9 can be activated without proteolytic processing. Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS. J Biol Chem. 1999 Mar 26. 274(13):8359-62. 10.1074/jbc.274.13.8359 PubMed 10085063