pET23b-Casp7 C186A-His
(Plasmid #11826)

• Depositing Lab: Guy Salvesen
• Publication: Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800.

Backbone

• Vector backbone: pET-23 b
• Backbone size w/o insert (bp): 3666
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caspase 7 catalytic mutant
• Alt name: Caspase 7
• Species: H. sapiens (human)
• Insert Size (bp): 1053
• Mutation: C186A
• Entrez Gene: CASP7 (a.k.a. CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3)
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: NdeI (unknown if destroyed)
• 5′ sequencing primer: T7 terminal primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry