pET23b-Casp3 C163A-His
(Plasmid #11822)

• Depositing Lab: Guy Salvesen
• Publication: Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-800.

Backbone

• Vector backbone: pET-23 b
• Backbone size w/o insert (bp): 3666
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caspase 3 catalytic mutant
• Alt name: Caspase 3
• Species: H. sapiens (human)
• Insert Size (bp): 999
• Mutation: C163A
• Entrez Gene: CASP3 (a.k.a. CPP32, CPP32B, SCA-1)
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: NdeI (unknown if destroyed)
• 5′ sequencing primer: T7 terminal primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry