pcDNA3-Casp9 C287A
(Plasmid #11819)

• Depositing Lab: Guy Salvesen
• Publication: Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-62.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Caspase 9 catalytically incompetent mutant
• Alt name: Caspase 9
• Species: H. sapiens (human)
• Insert Size (bp): 1392
• Mutation: C287A, catalytically incompetent mutant.
• Entrez Gene: CASP9 (a.k.a. APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Sanford-Burnham Medical Research Institute Ancillary Agreement
• Industry Terms:
  • Not Available to Industry