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Addgene

pcDNA3-Casp3-myc
(Plasmid #11813)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11813 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Caspase 3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    983
  • Entrez Gene
    CASP3 (a.k.a. CPP32, CPP32B, SCA-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer BGH-rev; Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Casp3-myc was a gift from Guy Salvesen (Addgene plasmid # 11813 ; http://n2t.net/addgene:11813 ; RRID:Addgene_11813)

  • For your References section:

    Biochemical characteristics of caspases-3, -6, -7, and -8. Stennicke HR, Salvesen GS. J Biol Chem. 1997 Oct 10. 272(41):25719-23. 10.1074/jbc.272.41.25719 PubMed 9325297
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