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pLX307 HRAS
(Plasmid #117748)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX307
  • Backbone manufacturer
    Broad Institute
  • Total vector size (bp) 8983
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RASH1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    509
  • Mutation
    Closed vector so will not read through the V5 region
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Promoter EF1a
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX307 HRAS was a gift from William Hahn (Addgene plasmid # 117748 ; http://n2t.net/addgene:117748 ; RRID:Addgene_117748)

  • For your References section:

    MCL1 and DEDD promote urothelial carcinoma progression. Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai A, Rosenberg JE, Hahn WC. Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 10.1158/1541-7786.MCR-18-0963. 10.1158/1541-7786.MCR-18-0963 PubMed 30777879
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