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Addgene

pNBU2_erm_P1T_DP-GH023 - NanoLuc
(Plasmid #117727)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNBU2-ErmG
  • Backbone size w/o insert (bp) 5097
  • Total vector size (bp) 5558
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EC100D pir-116
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NanoLuc
  • Insert Size (bp)
    516
  • GenBank ID
    AFI79290.1

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CCGCTTTCCAAGAGAAGAAAG
  • 3′ sequencing primer CACAATATGAGCAACAAGGAATCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNBU2_erm_P1T_DP-GH023 - NanoLuc was a gift from Andrew Goodman (Addgene plasmid # 117727 ; http://n2t.net/addgene:117727 ; RRID:Addgene_117727)

  • For your References section:

    Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut. Lim B, Zimmermann M, Barry NA, Goodman AL. Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045. 10.1016/j.cell.2017.03.045 PubMed 28431252
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