CMV BARK D110A p2A mCherry
(Plasmid
#117690)
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PurposeCMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 117690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA 3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBARKrgs D110A peptide
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Alt nameCMV-iBARK(D110A)-p2A-mCherry:
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SpeciesB. taurus (bovine)
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Insert Size (bp)1254
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MutationD110A
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Tag
/ Fusion Protein
- HA / FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV BARK D110A p2A mCherry was a gift from Baljit Khakh (Addgene plasmid # 117690 ; http://n2t.net/addgene:117690 ; RRID:Addgene_117690) -
For your References section:
Specific and behaviorally consequential astrocyte Gq GPCR signaling attenuation in vivo with ibetaARK. Nagai J, Bellafard A, Qu Z, Yu X, Ollivier M, Gangwani MR, Diaz-Castro B, Coppola G, Schumacher SM, Golshani P, Gradinaru V, Khakh BS. Neuron. 2021 Jul 21;109(14):2256-2274.e9. doi: 10.1016/j.neuron.2021.05.023. Epub 2021 Jun 16. 10.1016/j.neuron.2021.05.023 PubMed 34139149