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Addgene

pKar2-msGFP-HDEL
(Plasmid #117668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pKar2-msGFP-HDEL
  • Mutation
    Contains the Kar2 promoter and signal sequence from S.cerevisiae as well as a monomeric superfolder variant of GFP (msGFP) with the amino acids HDEL at the C-terminal.
  • Promoter pKar2

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Integration plasmid in the His4 locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKar2-msGFP-HDEL was a gift from Pau Ferrer & Benjamin Glick (Addgene plasmid # 117668 ; http://n2t.net/addgene:117668 ; RRID:Addgene_117668)

  • For your References section:

    An improved secretion signal enhances the secretion of model proteins from Pichia pastoris. Barrero JJ, Casler JC, Valero F, Ferrer P, Glick BS. Microb Cell Fact. 2018 Oct 12;17(1):161. doi: 10.1186/s12934-018-1009-5. 10.1186/s12934-018-1009-5 PubMed 30314480
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