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pPICZ-pre-Ost1-pro-alphaf-Btl2
(Plasmid #117665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZalpha
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pre-Ost1-pro-alphaf-Btl2
  • Mutation
    This plasmid encodes the lipase Btl2 together with an hibrid secretion signal. This secretion signal is composed by the the signal sequence of Ost1 from Saccharomyces cerevisiae and the the native mating alpha factor pro region from Saccharomyces cerevisiae. The pro region has Leucine at position 42.
  • Promoter pAOX1

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Integration plasmid in the pAOX1 locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPICZ-pre-Ost1-pro-alphaf-Btl2 was a gift from Pau Ferrer & Benjamin Glick (Addgene plasmid # 117665 ; http://n2t.net/addgene:117665 ; RRID:Addgene_117665)

  • For your References section:

    An improved secretion signal enhances the secretion of model proteins from Pichia pastoris. Barrero JJ, Casler JC, Valero F, Ferrer P, Glick BS. Microb Cell Fact. 2018 Oct 12;17(1):161. doi: 10.1186/s12934-018-1009-5. 10.1186/s12934-018-1009-5 PubMed 30314480
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