pPICZ-pre-pro-alphaf(I)-E2-Crimson
(Plasmid #117660)

• Purpose: Test intracellular localisation of E2-Crimson and study the secretion efficiency
• Depositing Labs: Pau Ferrer, Benjamin Glick
• Publication: Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161. doi: 10.1186/s12934-018-1009-5.

Backbone

• Vector backbone: pPICZalpha
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pre-pro-alphaf(I)-E2-Crimson
• Mutation: This plasmid encodes the fluorescent protein E2-Crimson together with the mating alpha factor secretion signal from Saccharomyces cerevisiae commonly seen in plasmid from Invitrogen. This secretion signal variant has Ser at position 42 and a trio of point mutations that create an XhoI restriction site while changing Asp83 to Glu in comparison with the native signal sequence.
• Promoter: pAOX1

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pre-pro-af(I)-E2-Crimson.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Integration plasmid in the pAOX1 locus.