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Addgene

STOP-eGFP-ROSA26TV
(Plasmid #11739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSII KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Cre/Lox ; /STOP
  • Selectable markers
    Neomycin (select with G418) ; Diptheriatoxin A

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STOP-eGFP-ROSA26TV
  • Species
    DNA derived from yeast E. Coli, transposon Tn5, Corynebacterium Aequoria (jellyfish)
  • Insert Size (bp)
    11500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7/M13
  • 3′ sequencing primer T7/M13
    • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Based on pRosa26-1 from Soriano P, Fred Hutchinson, Cancer Research Institute
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Targeting vector for cDNA knock-in into the Rosa26 locus.

See more information about the creation of this plasmid on the PDF attached to the CTV vector page http://www.addgene.org/15912

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STOP-eGFP-ROSA26TV was a gift from Klaus Rajewsky (Addgene plasmid # 11739 ; http://n2t.net/addgene:11739 ; RRID:Addgene_11739)

  • For your References section:

    Canonical NF-kappaB activity, dispensable for B cell development, replaces BAFF-receptor signals and promotes B cell proliferation upon activation. Sasaki Y, Derudder E, Hobeika E, Pelanda R, Reth M, Rajewsky K, Schmidt-Supprian M. Immunity. 2006 Jun . 24(6):729-39. 10.1016/j.immuni.2006.04.005 PubMed 16782029
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