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Addgene

pCMV5B-Flag-Smad1
(Plasmid #11735)

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Item Catalog # Description Quantity Price (USD)
Plasmid 11735 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV5B
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMAD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    NM_005900
  • Entrez Gene
    SMAD1 (a.k.a. BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5B-Flag-Smad1 was a gift from Jeff Wrana (Addgene plasmid # 11735 ; http://n2t.net/addgene:11735 ; RRID:Addgene_11735)

  • For your References section:

    MADR1, a MAD-related protein that functions in BMP2 signaling pathways. Hoodless PA, Haerry T, Abdollah S, Stapleton M, O'Connor MB, Attisano L, Wrana JL. Cell. 1996 May 17. 85(4):489-500. 10.1016/S0092-8674(00)81250-7 PubMed 8653785
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