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Addgene

pET-Gate2 mazEF
(Plasmid #117117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 117117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-Gate2
  • Backbone size (bp) 6400
  • Vector type
    Backbone for Golden gate assembly for gene targeting in Bacillus subtilis

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone vector for reconstitution of gene targeting constructs by Golden gate assembly. The MazEF toxin-antitoxin system couter-selects for single cross-over events that leads to incorporation of the vector backbone into the genome. Overhangs generated upon BsaI cleavage : CGAG (RU) and CCAT (RD).

(Gruber lab reference pSG1525)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Gate2 mazEF was a gift from Stephan Gruber (Addgene plasmid # 117117 ; http://n2t.net/addgene:117117 ; RRID:Addgene_117117)

  • For your References section:

    High-Throughput Allelic Replacement Screening in Bacillus subtilis. Diebold-Durand ML, Burmann F, Gruber S. Methods Mol Biol. 2019;2004:49-61. doi: 10.1007/978-1-4939-9520-2_5. 10.1007/978-1-4939-9520-2_5 PubMed 31147909
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