H2B-GFP
(Plasmid #11680)

• Depositing Lab: Geoff Wahl
• Publication: Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human histone H2B
• Species: H. sapiens (human)
• Insert Size (bp): 380
• GenBank ID: X00088
• Entrez Gene: H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest for 11680
• Articles Citing this Plasmid:
  • 94 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Two sequence differences from consensus sequence (D26G and V119I) were detected in Addgene sequencing. This clone is as originally published and isolated.