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Addgene

pIND-GFP
(Plasmid #11612)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIND
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression ; Ecdysone Inducible
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green Fluorescent Protein
  • Alt name
    GFP
  • Insert Size (bp)
    600

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer see pIND
  • 3′ sequencing primer see pIND
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a GFP inducible gene construct....inducible with pronosterone or other analogs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIND-GFP was a gift from Steven Johnson (Addgene plasmid # 11612 ; http://n2t.net/addgene:11612 ; RRID:Addgene_11612)
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