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Addgene

pKISox1-P2A-eGFP-Hygro
(Plasmid #115685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    P2A-eGFP-loxP-PGK-Hygro-loxP
  • Species
    Synthetic
  • Insert Size (bp)
    2719

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PGK-Hygromycin can be removed by Cre

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKISox1-P2A-eGFP-Hygro was a gift from David Suter (Addgene plasmid # 115685 ; http://n2t.net/addgene:115685 ; RRID:Addgene_115685)

  • For your References section:

    A role for mitotic bookmarking of SOX2 in pluripotency and differentiation. Deluz C, Friman ET, Strebinger D, Benke A, Raccaud M, Callegari A, Leleu M, Manley S, Suter DM. Genes Dev. 2016 Nov 15;30(22):2538-2550. doi: 10.1101/gad.289256.116. Epub 2016 Dec 5. 10.1101/gad.289256.116 PubMed 27920086
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