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Addgene

pGL001
(Plasmid #115674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Total vector size (bp) 4545
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    fliC promoter fused with GFP
  • Alt name
    pfliC - GFP
  • Species
    Escherichia coli
  • Insert Size (bp)
    896
  • GenBank ID
  • Promoter fliC promoter
  • Tag / Fusion Protein
    • Green fluorescent protein (GFP) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTATTACAGGATTAATAGCGGGAATAAGG
  • 3′ sequencing primer CAAGCTTCTTAGCTTGCATGCGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL001 was a gift from Gene-Wei Li (Addgene plasmid # 115674 ; http://n2t.net/addgene:115674 ; RRID:Addgene_115674)

  • For your References section:

    Rapid Accumulation of Motility-Activating Mutations in Resting Liquid Culture of Escherichia coli. Parker DJ, Demetci P, Li GW. J Bacteriol. 2019 Sep 6;201(19). pii: JB.00259-19. doi: 10.1128/JB.00259-19. Print 2019 Oct 1. 10.1128/JB.00259-19 PubMed 31285239
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