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Addgene

pCDNA3-FLAG-SENP3-C526A
(Plasmid #115522)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115522 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SENP3-C526A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Mutation
    C526A
  • Entrez Gene
    Senp3 (a.k.a. AA408656, Smt3ip, Smt3ip1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-FLAG-SENP3-C526A was a gift from Martine Roussel (Addgene plasmid # 115522 ; http://n2t.net/addgene:115522 ; RRID:Addgene_115522)

  • For your References section:

    Arf-induced turnover of the nucleolar nucleophosmin-associated SUMO-2/3 protease Senp3. Kuo ML, den Besten W, Thomas MC, Sherr CJ. Cell Cycle. 2008 Nov 1;7(21):3378-87. doi: 10.4161/cc.7.21.6930. Epub 2008 Nov 4. 10.4161/cc.7.21.6930 PubMed 18948745
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