pZ P (cHS4)X4 OCT4p eGFP SV40pA v2
(Plasmid
#115518)
-
PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepZ F3 CAGGS GPHTK F (AddGene plasmid 112666)
- Backbone size w/o insert (bp) 9000
- Total vector size (bp) 9820
-
Vector typeMammalian Expression, AAV ; Donor plasmid for recombinase-mediated cassette exchange
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameeGFP
-
Alt nameGFP
-
Insert Size (bp)717
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pZ P (cHS4)X4 OCT4p eGFP SV40pA v2 was a gift from Catherine Verfaillie (Addgene plasmid # 115518 ; http://n2t.net/addgene:115518 ; RRID:Addgene_115518) -
For your References section:
Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition. Ordovas L, Boon R, Pistoni M, Chen Y, Wolfs E, Guo W, Sambathkumar R, Bobis-Wozowicz S, Helsen N, Vanhove J, Berckmans P, Cai Q, Vanuytsel K, Eggermont K, Vanslembrouck V, Schmidt BZ, Raitano S, Van Den Bosch L, Nahmias Y, Cathomen T, Struys T, Verfaillie CM. Stem Cell Reports. 2015 Nov 10;5(5):918-931. doi: 10.1016/j.stemcr.2015.09.004. Epub 2015 Oct 8. 10.1016/j.stemcr.2015.09.004 PubMed 26455413
Map uploaded by the depositor.
