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Addgene

pCAG-Bgh-GFP-Farpl
(Plasmid #115501)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115501 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-BGH
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Farp1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Farp1
  • Promoter chicken beta-actin
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Bgh-GFP-Farpl was a gift from Thomas Biederer (Addgene plasmid # 115501 ; http://n2t.net/addgene:115501 ; RRID:Addgene_115501)

  • For your References section:

    The novel synaptogenic protein Farp1 links postsynaptic cytoskeletal dynamics and transsynaptic organization. Cheadle L, Biederer T. J Cell Biol. 2012 Dec 10;199(6):985-1001. doi: 10.1083/jcb.201205041. Epub 2012 Dec 3. 10.1083/jcb.201205041 PubMed 23209303
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