pEYFP-N1-eKR2
(Plasmid #115337)

• Purpose: Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse
• Depositing Lab: Peter Hegemann
• Publication: Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.1038/s41598-018-27690-w.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 3994
• Total vector size (bp): 5773
• Modifications to backbone: EGFP was replaced by construct with tag and targeting sequences between BamHI and XbaI
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eKR2
• Species: Synthetic; Dokdonia eikasta, Chlamydomonas reinhardtii
• Mutation: none
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: pEGFP-N1_F (GAGGTCTATATAAGCAGAGC), CMV_F (GCAAATGGGCGGTAGGCGT)
• 3′ sequencing primer: pEGFP-C1_R (AACCATTATAAGCTGCAATAAAC)

Resource Information

• Supplemental Documents:
  • pEYFP-N1-eKR2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry