pGEM-mouse TCR zeta/2451
(Plasmid
#11506)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-3
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3200
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Vector typegeneral cloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTCR zeta
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SpeciesM. musculus (mouse)
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Insert Size (bp)1300
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Mutationn/a
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GenBank IDM19729
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Entrez GeneCd247 (a.k.a. 4930549J05Rik, A430104F18Rik, Cd3, Cd3-eta, Cd3-zeta, Cd3h, Cd3z, Cd3zeta, T3z, Tcrk, Tcrz)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR I (not destroyed)
- 3′ cloning site EcoR I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-mouse TCR zeta/2451 was a gift from Allan Weissman (Addgene plasmid # 11506 ; http://n2t.net/addgene:11506 ; RRID:Addgene_11506) -
For your References section:
Molecular cloning of the zeta chain of the T cell antigen receptor. Weissman AM, Baniyash M, Hou D, Samelson LE, Burgess WH, Klausner RD. Science. 1988 Feb 26. 239(4843):1018-21. 10.1126/science.3278377 PubMed 3278377