iLID-eGFP-2xC2
(Plasmid
#114416)
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Purposeanchor iLID domain to plasma membrane with slower diffusion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114416 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiLID domain
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SpeciesSynthetic
- Promoter CMV
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Tag
/ Fusion Protein
- eGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iLID-eGFP-2xC2 was a gift from Dorus Gadella (Addgene plasmid # 114416 ; http://n2t.net/addgene:114416 ; RRID:Addgene_114416) -
For your References section:
Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Van Geel O, Hartsuiker R, Gadella TWJ. Small GTPases. 2018 Sep 5:1-10. doi: 10.1080/21541248.2018.1507411. 10.1080/21541248.2018.1507411 PubMed 30182785
