pPN10-gCTG
(Plasmid
#114385)
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PurposepPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114385 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPN10
- Backbone size w/o insert (bp) 5257
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegCTG
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gRNA/shRNA sequence(CTG)6 gRNA
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Insert Size (bp)5316
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPN10-gCTG was a gift from Vincent Dion (Addgene plasmid # 114385 ; http://n2t.net/addgene:114385 ; RRID:Addgene_114385) -
For your References section:
Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase. Cinesi C, Aeschbach L, Yang B, Dion V. Nat Commun. 2016 Nov 9;7:13272. doi: 10.1038/ncomms13272. 10.1038/ncomms13272 PubMed 27827362