Skip to main content
Addgene

AAV-CamKIIa-ChR2-EGFP
(Plasmid #114216)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 114216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    AAV
  • Total vector size (bp) 7079
  • Vector type
    Mammalian Expression, Mouse Targeting, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Channelrhodopsin 2
  • Alt name
    ChR2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1665
  • Promoter CamKIIa 1.3 kb
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied sequence. The authors have noted that the discrepancies do NOT affect plasmid function or viral packaging.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CamKIIa-ChR2-EGFP was a gift from Xue Han (Addgene plasmid # 114216 ; http://n2t.net/addgene:114216 ; RRID:Addgene_114216)

  • For your References section:

    A MicroRNA-Based Gene-Targeting Tool for Virally Labeling Interneurons in the Rodent Cortex. Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X. Cell Rep. 2018 Jul 10;24(2):294-303. doi: 10.1016/j.celrep.2018.06.049. 10.1016/j.celrep.2018.06.049 PubMed 29996091
Related items:
Commonly requested with: