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PurposeTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113862 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMuLE Lenti Dest Neo
- Backbone size w/o insert (bp) 9237
- Total vector size (bp) 15489
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418) ; eGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCMV-eGFP
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Insert Size (bp)1475
Cloning Information for Gene/Insert 1
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTOP-NLuc1.1
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Alt nameM50 Super 8x TOPFlash, promoter only
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Alt nameNL1.1
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Insert Size (bp)822
Cloning Information for Gene/Insert 2
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert name12GLI-Fluc
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Alt namefirefly luciferase
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Insert Size (bp)4136
Cloning Information for Gene/Insert 3
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameCBF-GLuc
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Alt nameCBFRE
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Alt nameGaussia luciferase
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Insert Size (bp)1090
Cloning Information for Gene/Insert 4
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBackbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc was a gift from Manfred Ogris (Addgene plasmid # 113862 ; http://n2t.net/addgene:113862 ; RRID:Addgene_113862) -
For your References section:
Luminescent and fluorescent triple reporter plasmid constructs for Wnt, Hedgehog and Notch pathway. Maier J, Elmenofi S, Taschauer A, Anton M, Sami H, Ogris M. PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019. 10.1371/journal.pone.0226570 PubMed 31860685