pMuLE_ENTR_12GLI-FLuc_R4-R3
(Plasmid #113712)

• Purpose: Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.
• Depositing Lab: Manfred Ogris
• Publication: Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. doi: 10.1371/journal.pone.0226570. eCollection 2019.

Backbone

• Vector backbone: pMuLE ENTR MCS R4-R3
• Backbone size w/o insert (bp): 2775
• Total vector size (bp): 6801
• Vector type: Mammalian Expression ; MuLE Gateway Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 12GLI-FLuc
• Insert Size (bp): 4034

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: M13

Resource Information

• Supplemental Documents:
  • pMuLE_ENTR_12GLI-FLuc_R4-R3.gb
  • Plasmid History
• A portion of this plasmid was derived from a plasmid made by: 12GLI-RETKO-luc was a gift from Peter Zaphiropoulos. Backbone is included in the MuLE kit, which was a gift from Ian Frew (Addgene kit #1000000060).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Citation for 12GLI-RETKO-luc: Kogerman P, Grimm T, Kogerman L, Krause D, Undén AB, Sandstedt B, et al. Mammalian suppressor-of-fused modulates nuclear–cytoplasmic shuttling of Gli-1. Nature cell biology. 1999;1(5):312.