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Addgene

BK alpha subunit
(Plasmid #113566)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    BK alpha includes the MANG (full length) amino terminus
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kcnma1
  • Alt name
    alpha1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4000
  • Entrez Gene
    Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BK alpha subunit was a gift from Robert Brenner (Addgene plasmid # 113566 ; http://n2t.net/addgene:113566 ; RRID:Addgene_113566)

  • For your References section:

    An extracellular domain of the accessory beta1 subunit is required for modulating BK channel voltage sensor and gate. Gruslova A, Semenov I, Wang B. J Gen Physiol. 2012 Jan;139(1):57-67. doi: 10.1085/jgp.201110698. Epub 2011 Dec 12. 10.1085/jgp.201110698 PubMed 22155735

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