nud-1::L4440
(Plasmid #11337)

• Depositing Lab: Guy Caldwell
• Publication: Integrated Genomics (unpublished)

Backbone

• Vector backbone: L4440
• Backbone manufacturer: L4440 is available at Addgene
• Backbone size w/o insert (bp): 2790
• Vector type: Worm Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nud-1
• Species: C. elegans (nematode)
• Insert Size (bp): 963
• GenBank ID: NM_067348
• Entrez Gene: nud-1 (a.k.a. CELE_F53A2.4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: L4440 primer (AGCGAGTCAGTGAGCGAG)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Andrew Fire created the vector backbone L4440

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.