pJY3919
(Plasmid #113332)

• Purpose: Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter
• Depositing Lab: Suzanne Sandmeyer
• Publication: Patterson et al Metab Eng. 2018 May 22;48:184-196. doi: 10.1016/j.ymben.2018.05.008.

Backbone

• Vector backbone: pPS3911
• Total vector size (bp): 11503
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: beta-isopropyl malate dehydrogenase
• Alt name: LEU2
• Species: Yarrowia lipolytica
• Insert Size (bp): 2087

Gene/Insert 2

• Gene/Insert name: codon-optimized Hermes transposase
• Species: Yarrowia lipolytica
• Insert Size (bp): 1863

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Plasmid contains a T base insertion near the C-terminus of the Leu2 marker. This insertion is not known to affect Leu2 function.