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PurposeOmomyc expression in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS+
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Backbone manufacturerDavid Turner
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOmomyc
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SpeciesH. sapiens (human)
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Insert Size (bp)300
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MutationE57T, E64I, R70Q, R71N
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS Omomyc was a gift from Sergio Nasi (Addgene plasmid # 113164 ; http://n2t.net/addgene:113164 ; RRID:Addgene_113164) -
For your References section:
Design and properties of a Myc derivative that efficiently homodimerizes. Soucek L, Helmer-Citterich M, Sacco A, Jucker R, Cesareni G, Nasi S. Oncogene. 1998 Nov 12;17(19):2463-72. doi: 10.1038/sj.onc.1202199. 10.1038/sj.onc.1202199 PubMed 9824157