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pSumo24P7vB4
(Plasmid #113073)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    2% glucose during propagation of plasmid is highly recommended. Accumulation of mutations was observed.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag
  • Alt name
    IL-24
  • Alt name
    IL-24/MDA7
  • Species
    H. sapiens (human); SUMO part (Brachypodium distachyon)
  • Insert Size (bp)
    831
  • Mutation
    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q94A, T111Q, E114R, K118D, R127K, V129A, V131L, Q150D, S154C, N157Q, Q158N, F160C, I162S, D164E, S165E, L172Q, R174Q, K178E, L186A, V193I, K205Q
  • Entrez Gene
    IL24 (a.k.a. C49A, FISP, IL10B, MDA7, MOB5, ST16)
  • Promoter T7
  • Tag / Fusion Protein
    • SUMO (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pET upstream primer
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSumo24P7vB4 was a gift from Bohdan Schneider (Addgene plasmid # 113073 ; http://n2t.net/addgene:113073 ; RRID:Addgene_113073)

  • For your References section:

    Flexible regions govern promiscuous binding of IL-24 to receptors IL-20R1 and IL-22R1. Zahradnik J, Kolarova L, Peleg Y, Kolenko P, Svidenska S, Charnavets T, Unger T, Sussman JL, Schneider B. FEBS J. 2019 Oct;286(19):3858-3873. doi: 10.1111/febs.14945. Epub 2019 Jun 12. 10.1111/febs.14945 PubMed 31152679
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