pSumo24P7
(Plasmid
#113072)
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PurposePlasmid for highly efficient expression of engineered IL24 with mutated binding sites
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113072 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructions2% glucose during propagation of plasmid is highly recommended. Accumulation of mutations was observed.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHuman IL-24 engineered by computational design and fused with N-terminal SUMO tag
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Alt nameIL-24
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Alt nameIL-24/MDA7
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SpeciesH. sapiens (human); SUMO part (Brachypodium distachyon)
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Insert Size (bp)831
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MutationQ60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q94A, T111Q, E114R, K118D, R127K, V129A, V131L, V145L, L146Q, V148R, S149K, Q150D, S154C, N157Q, Q158N, F160C, I162S, D164E, S165E, L172Q, R174Q, K178E, L186A, V193I, T198R, K205Q
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Entrez GeneIL24 (a.k.a. C49A, FISP, IL10B, MDA7, MOB5, ST16)
- Promoter T7
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Tag
/ Fusion Protein
- SUMO (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pET upstream primer
- 3′ sequencing primer T7 terminator primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSumo24P7 was a gift from Bohdan Schneider (Addgene plasmid # 113072 ; http://n2t.net/addgene:113072 ; RRID:Addgene_113072) -
For your References section:
Flexible regions govern promiscuous binding of IL-24 to receptors IL-20R1 and IL-22R1. Zahradnik J, Kolarova L, Peleg Y, Kolenko P, Svidenska S, Charnavets T, Unger T, Sussman JL, Schneider B. FEBS J. 2019 Oct;286(19):3858-3873. doi: 10.1111/febs.14945. Epub 2019 Jun 12. 10.1111/febs.14945 PubMed 31152679