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Addgene

pGex4t2 with full-length SUUR
(Plasmid #112990)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112990 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX 4t2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuUR
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    SuUR (a.k.a. Dmel_CG7869, CG7869, Dmel\CG7869, Dromel_CG7869_FBtr0076203_suur_mORF, SU(UR), SUUR, Su(UR), Su(UR)ES, su(UR)ES)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGex4t2 with full-length SUUR was a gift from Terry Orr-Weaver (Addgene plasmid # 112990 ; http://n2t.net/addgene:112990 ; RRID:Addgene_112990)
  • For your References section:

    DNA copy-number control through inhibition of replication fork progression. Nordman JT, Kozhevnikova EN, Verrijzer CP, Pindyurin AV, Andreyeva EN, Shloma VV, Zhimulev IF, Orr-Weaver TL. Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10.1016/j.celrep.2014.10.005. Epub 2014 Oct 30. 10.1016/j.celrep.2014.10.005 PubMed 25437540