pGex4t2 with full-length SUUR
(Plasmid
#112990)
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PurposeExpresses fusion of GST and full-length Drosophila SUUR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX 4t2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuUR
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SpeciesD. melanogaster (fly)
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Entrez GeneSuUR (a.k.a. Dmel_CG7869, CG7869, Dmel\CG7869, Dromel_CG7869_FBtr0076203_suur_mORF, SU(UR), SUUR, Su(UR), Su(UR)ES, su(UR)ES)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site SmaI (destroyed during cloning)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGex4t2 with full-length SUUR was a gift from Terry Orr-Weaver (Addgene plasmid # 112990 ; http://n2t.net/addgene:112990 ; RRID:Addgene_112990) -
For your References section:
DNA copy-number control through inhibition of replication fork progression. Nordman JT, Kozhevnikova EN, Verrijzer CP, Pindyurin AV, Andreyeva EN, Shloma VV, Zhimulev IF, Orr-Weaver TL. Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10.1016/j.celrep.2014.10.005. Epub 2014 Oct 30. 10.1016/j.celrep.2014.10.005 PubMed 25437540