pEv-Fat4-citrine
(Plasmid #112854)

• Purpose: Constitutive expression of human fat4 protein fused to citrine fluorescent protein
• Depositing Lab: David Sprinzak
• Publication: Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 10.7554/eLife.24820.

Backbone

• Vector backbone: pEv
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FAT4
• Alt name: FAT atypical cadherin 4
• Species: H. sapiens (human)
• Entrez Gene: FAT4 (a.k.a. CDHF14, CDHR11, FAT-J, FATJ, HKLLS2, NBLA00548, VMLDS2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Citrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pEV fat cit.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry